Alfa Cytology, a specialized biotechnology company headquartered in the United States, focusing on tumor microenvironment (TME), has announced its molecular biology services for TME research. The launch of the service indicates that the company is developing towards precision and depth in TME research. TME refers to the surrounding environment of tumor cells, which feed the latter as normal cells, molecules, and blood vessels. They make an influence on each other closely. Analyzing the biological components in TME helps to detect harmful substances in cells as early as possible, facilitating early detection, diagnosis, and treatment. Molecular biology tools offer valuable insights into gene expression patterns, protein profiles, genetic alterations, epigenetic modifications, and functional properties of key molecules within the TME. By employing techniques such as next-generation sequencing (NGS), single-cell sequencing, quantitative polymerase chain reaction (qPCR), and DNA